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mouse il  (R&D Systems)


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    R&D Systems mouse il
    Mouse Il, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+il+12+23+p40/bio_rxiv__64898__2026__01__29__702227-278-14-21?v=R%26D+Systems
    Average 94 stars, based on 22 article reviews
    mouse il - by Bioz Stars, 2026-08
    94/100 stars

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    Bone marrow–derived dendritic cells were treated with 2.5 µg of modified or unmodified mRNA encapsulated in LNPs, with an equivalent amount of empty LNPs, or with vehicle (water). Representative histograms show the percentage of CD40-CD80-CD86-CCR7, and MHCII positive cells assessed by flow cytometry, and IL-12 concentrations measured by ELISA ± SEM at 24 or 48 h post treatment (n = 9 at 24 h; n = 3 at 48 h). ***p < 0.001 versus vehicle (two-way ANOVA, Dunnett’s test).

    Journal: bioRxiv

    Article Title: Spatiotemporal Atlas of Pro-Inflammatory (NF-κB) and Anti-Inflammatory (STAT6) Signalling Using Reporter Mice during mRNA Vaccination

    doi: 10.64898/2026.01.29.702227

    Figure Lengend Snippet: Bone marrow–derived dendritic cells were treated with 2.5 µg of modified or unmodified mRNA encapsulated in LNPs, with an equivalent amount of empty LNPs, or with vehicle (water). Representative histograms show the percentage of CD40-CD80-CD86-CCR7, and MHCII positive cells assessed by flow cytometry, and IL-12 concentrations measured by ELISA ± SEM at 24 or 48 h post treatment (n = 9 at 24 h; n = 3 at 48 h). ***p < 0.001 versus vehicle (two-way ANOVA, Dunnett’s test).

    Article Snippet: The concentration of IL-12 in supernatants from BM-DCs was determined by ELISA using the Mouse IL-12/IL-23 p40 Allele-specific DuoSet ELISA kit (R&D Systems, Cat. DY499) following the manufacturer’s instructions.

    Techniques: Derivative Assay, Modification, Flow Cytometry, Enzyme-linked Immunosorbent Assay

    (A, C) Representative ex vivo bioluminescence images of organs dissected from NFκB -luc2 and STAT6 -luc2 reporter mice at (A) 5 hours or (C) 29 days after treatment. Signals are shown using pseudocolors according to the scale bar. Quantification of organ bioluminescence is shown in the bar graphs, representing mean photon emission (photons/s/cm²/sr) ± SEM (n = 6). *p < 0.05, **p < 0.01, ** p < 0.001 versus LNP (two-way ANOVA, Dunnett’s test).Thym: thymus, LN: inguinal lymph nodes, Dued.: duodenum. (B, D) Cytokine levels in plasma were measured (B) 5 hours after administration or (D) at day 29. Cytokine concentrations are presented as bar graphs ± SEM (n = 3). *p < 0.05, * p < 0.01 versus LNP (one-way ANOVA, Dunnett’s test). (E) Antigen-specific antibodies were measured in plasma at day 29 by ELISA. Bar graphs represent mean absorbance ± SEM (n = 12). ** p < 0.001 versus LNP (two-way ANOVA, Dunnett’s test).

    Journal: bioRxiv

    Article Title: Spatiotemporal Atlas of Pro-Inflammatory (NF-κB) and Anti-Inflammatory (STAT6) Signalling Using Reporter Mice during mRNA Vaccination

    doi: 10.64898/2026.01.29.702227

    Figure Lengend Snippet: (A, C) Representative ex vivo bioluminescence images of organs dissected from NFκB -luc2 and STAT6 -luc2 reporter mice at (A) 5 hours or (C) 29 days after treatment. Signals are shown using pseudocolors according to the scale bar. Quantification of organ bioluminescence is shown in the bar graphs, representing mean photon emission (photons/s/cm²/sr) ± SEM (n = 6). *p < 0.05, **p < 0.01, ** p < 0.001 versus LNP (two-way ANOVA, Dunnett’s test).Thym: thymus, LN: inguinal lymph nodes, Dued.: duodenum. (B, D) Cytokine levels in plasma were measured (B) 5 hours after administration or (D) at day 29. Cytokine concentrations are presented as bar graphs ± SEM (n = 3). *p < 0.05, * p < 0.01 versus LNP (one-way ANOVA, Dunnett’s test). (E) Antigen-specific antibodies were measured in plasma at day 29 by ELISA. Bar graphs represent mean absorbance ± SEM (n = 12). ** p < 0.001 versus LNP (two-way ANOVA, Dunnett’s test).

    Article Snippet: The concentration of IL-12 in supernatants from BM-DCs was determined by ELISA using the Mouse IL-12/IL-23 p40 Allele-specific DuoSet ELISA kit (R&D Systems, Cat. DY499) following the manufacturer’s instructions.

    Techniques: Ex Vivo, Clinical Proteomics, Enzyme-linked Immunosorbent Assay